中国水稻科学 ›› 2017, Vol. 31 ›› Issue (1): 31-39.DOI: 10.16819/j.1001-7216.2017.6046
张杰, 郑蕾娜, 蔡跃, 尤小满, 孔飞, 汪国湘, 燕海刚, 金洁, 王亮, 张文伟*(), 江玲
收稿日期:
2016-03-17
修回日期:
2016-04-09
出版日期:
2017-01-20
发布日期:
2017-01-10
通讯作者:
张文伟
基金资助:
Jie ZHANG, Leina ZHENG, Yue CAI, Xiaoman YOU, Fei KONG, Guoxiang WANG, Haigang YAN, Jie JIN, Liang WANG, Wenwei ZHANG*(), Ling JIANG
Received:
2016-03-17
Revised:
2016-04-09
Online:
2017-01-20
Published:
2017-01-10
Contact:
Wenwei ZHANG
摘要:
目的 检测到新的控制稻米品质性状相关的QTL并分析各性状间的相关性,为了解控制水稻品质的遗传机理和培育优质水稻品种奠定基础。方法 利用Sasanishiki×Habataki回交重组自交系(backcross inbred lines, BILs)群体在两个环境下种植的结果,检测与稻米直链淀粉含量、蛋白质含量及RVA谱特征值相关的加性QTL。结果 表型分析结果显示,Habataki的蛋白含量明显高于Sasanishiki;而除消减值以外其余的稻米品质性状指标,Sasanishiki均高于Habataki。利用BIL群体共检测到加性QTL 42个,其中10个QTL位点在2个环境中均能被检测到,即qPC8、qAC4、qAC10、qPKV2、qPKV7、qHPV7、qCPV1、qBDV4、qBDV7、qSBV7,且qCPV1、qBDV4、qPKV7、qHPV7和qAC10等5个QTL尚未见报道。同时,我们还利用Sasanishiki×Habataki染色体片断置换系(Chromosome segment substitution lines, CSSLs)验证了10个稳定表达的QTL位点。结论 稻米RVA谱特征值与直链淀粉含量、蛋白质含量之间呈现一定相关性,且控制不同品质性状的QTL之间具有共定位现象。
张杰, 郑蕾娜, 蔡跃, 尤小满, 孔飞, 汪国湘, 燕海刚, 金洁, 王亮, 张文伟, 江玲. 稻米淀粉RVA谱特征值与直链淀粉、蛋白含量的相关性及QTL定位分析[J]. 中国水稻科学, 2017, 31(1): 31-39.
Jie ZHANG, Leina ZHENG, Yue CAI, Xiaoman YOU, Fei KONG, Guoxiang WANG, Haigang YAN, Jie JIN, Liang WANG, Wenwei ZHANG, Ling JIANG. Correlation Analysis and QTL Mapping for Starch RVA Profile Properties and Amylose and Protein Contents in Rice[J]. Chinese Journal OF Rice Science, 2017, 31(1): 31-39.
性状 Trait | 亲本(平均值±标准差) Parent(Mean±SD) | BIL群体 BIL population | |||
---|---|---|---|---|---|
平均值±标准差 Mean±SD | 变异范围 Range | ||||
Sasanishiki | Habataki | ||||
蛋白质含量 Protein content/% | 7.15±0.01 | 8.09±0.02 | 7.96±0.78 | 6.52~9.94 | |
7.36±0.02 | 8.79±0.01 | 7.96±0.75 | 6.55~9.78 | ||
直链淀粉含量 Amylose content/% | 14.18±0.11 | 12.89±0.22 | 13.09±2.14 | 9.58~24.87 | |
14.32±0.12 | 13.04±0.20 | 13.08±2.12 | 9.65~24.95 | ||
峰值黏度 Peak viscosity/BU | 362.75±12.00 | 222.58±12.80 | 345.86±35.90 | 167.67~396.33 | |
301.83±4.00 | 279.25±3.00 | 299.32±21.89 | 244.50~349.25 | ||
热浆黏度 Hot paste viscosity/BU | 229.75±8.00 | 102.42±6.90 | 206.62±25.30 | 134.75~267.08 | |
134.58±4.50 | 121.42±6.00 | 324.02±52.02 | 106.75~179.50 | ||
崩解值 Breakdown viscosity/BU | 133.00±4.00 | 120.17±7.20 | 139.24±24.30 | 32.92~186.75 | |
167.25±0.50 | 157.83±3.00 | 212.26±120.06 | 114.50~186.58 | ||
冷胶黏度 Cool paste viscosity/BU | 326.67±20.20 | 178.67±9.30 | 298.16±23.77 | 241.08~352.67 | |
235.75±1.50 | 225.33±1.00 | 416.30±53.47 | 202.42~314.67 | ||
消减值 Setback viscosity/BU | -36.08±8.10 | -43.92±3.50 | -47.70±35.24 | -119.58~134.25 | |
-66.08±5.50 | -53.92±2.00 | -120.17±113.11 | -108.00~29.50 | ||
回复值 Consistence viscosity/BU | 96.92±12.20 | 76.25±2.40 | 91.54±14.86 | 61.66~167.17 | |
107.17±6.00 | 103.91±5.00 | 92.28±39.78 | 62.50~144.00 | ||
峰值时间 Peak time/min | 6.27±0.03 | 5.73±0.08 | 6.21±0.18 | 5.73~6.53 | |
5.87±0.16 | 5.67±0.10 | 5.91±0.42 | 5.25~6.83 |
表1 BIL群体及其亲本的品质性状在两个环境中的表型变异
Table 1 Descriptive statistics of the rice quality traits(parameters) in parents and BIL population in two environments.
性状 Trait | 亲本(平均值±标准差) Parent(Mean±SD) | BIL群体 BIL population | |||
---|---|---|---|---|---|
平均值±标准差 Mean±SD | 变异范围 Range | ||||
Sasanishiki | Habataki | ||||
蛋白质含量 Protein content/% | 7.15±0.01 | 8.09±0.02 | 7.96±0.78 | 6.52~9.94 | |
7.36±0.02 | 8.79±0.01 | 7.96±0.75 | 6.55~9.78 | ||
直链淀粉含量 Amylose content/% | 14.18±0.11 | 12.89±0.22 | 13.09±2.14 | 9.58~24.87 | |
14.32±0.12 | 13.04±0.20 | 13.08±2.12 | 9.65~24.95 | ||
峰值黏度 Peak viscosity/BU | 362.75±12.00 | 222.58±12.80 | 345.86±35.90 | 167.67~396.33 | |
301.83±4.00 | 279.25±3.00 | 299.32±21.89 | 244.50~349.25 | ||
热浆黏度 Hot paste viscosity/BU | 229.75±8.00 | 102.42±6.90 | 206.62±25.30 | 134.75~267.08 | |
134.58±4.50 | 121.42±6.00 | 324.02±52.02 | 106.75~179.50 | ||
崩解值 Breakdown viscosity/BU | 133.00±4.00 | 120.17±7.20 | 139.24±24.30 | 32.92~186.75 | |
167.25±0.50 | 157.83±3.00 | 212.26±120.06 | 114.50~186.58 | ||
冷胶黏度 Cool paste viscosity/BU | 326.67±20.20 | 178.67±9.30 | 298.16±23.77 | 241.08~352.67 | |
235.75±1.50 | 225.33±1.00 | 416.30±53.47 | 202.42~314.67 | ||
消减值 Setback viscosity/BU | -36.08±8.10 | -43.92±3.50 | -47.70±35.24 | -119.58~134.25 | |
-66.08±5.50 | -53.92±2.00 | -120.17±113.11 | -108.00~29.50 | ||
回复值 Consistence viscosity/BU | 96.92±12.20 | 76.25±2.40 | 91.54±14.86 | 61.66~167.17 | |
107.17±6.00 | 103.91±5.00 | 92.28±39.78 | 62.50~144.00 | ||
峰值时间 Peak time/min | 6.27±0.03 | 5.73±0.08 | 6.21±0.18 | 5.73~6.53 | |
5.87±0.16 | 5.67±0.10 | 5.91±0.42 | 5.25~6.83 |
指标 Index | 蛋白质含量 PC | 直链淀粉含量 AC | 峰值黏度 PKV | 热浆黏度 HPV | 崩解值 BDV | 冷胶黏度 CPV | 消减值 SBV | 回复值 CSV |
---|---|---|---|---|---|---|---|---|
直链淀粉含量 AC | -0.22 | |||||||
-0.14 | ||||||||
峰值黏度 PKV | -0.24 | -0.60** | ||||||
-0.17 | -0.20 | |||||||
热浆黏度 HPV | -0.36** | -0.23* | 0.74** | |||||
-0.39** | -0.15 | 0.73** | ||||||
崩解值 BDV | -0.29* | -0.64** | 0.71** | 0.05 | ||||
-0.26* | -0.54** | 0.70** | 0.03 | |||||
冷胶黏度 CPV | -0.38** | 0.26* | 0.36** | 0.82** | -0.32** | |||
-0.33** | 0.17 | 0.44** | 0.83** | -0.21 | ||||
消减值 SBV | 0.19 | 0.78** | -0.78** | -0.20 | -0.94** | 0.31** | ||
0.12 | 0.86** | -0.62** | -0.10 | -0.89** | 0.43** | |||
回复值 CSV | 0.02 | 0.81** | -0.68** | -0.40** | -0.60** | 0.21 | 0.83** | |
0.02 | 0.78** | -0.27* | -0.41** | -0.41** | 0.58** | 0.78** | ||
峰值时间 PeT | 0.07 | 0.46** | -0.17 | 0.45** | -0.72** | 0.69** | 0.64** | 0.34** |
0.02 | 0.38** | -0.27* | 0.41** | -0.41** | 0.58** | 0.78** | 0.92** |
表2 BIL群体及其亲本的品质性状在两种环境中的相关系数
Table 2 Coefficients of pairwise correlations of the rice quality traits in a BIL population observed in two environments.
指标 Index | 蛋白质含量 PC | 直链淀粉含量 AC | 峰值黏度 PKV | 热浆黏度 HPV | 崩解值 BDV | 冷胶黏度 CPV | 消减值 SBV | 回复值 CSV |
---|---|---|---|---|---|---|---|---|
直链淀粉含量 AC | -0.22 | |||||||
-0.14 | ||||||||
峰值黏度 PKV | -0.24 | -0.60** | ||||||
-0.17 | -0.20 | |||||||
热浆黏度 HPV | -0.36** | -0.23* | 0.74** | |||||
-0.39** | -0.15 | 0.73** | ||||||
崩解值 BDV | -0.29* | -0.64** | 0.71** | 0.05 | ||||
-0.26* | -0.54** | 0.70** | 0.03 | |||||
冷胶黏度 CPV | -0.38** | 0.26* | 0.36** | 0.82** | -0.32** | |||
-0.33** | 0.17 | 0.44** | 0.83** | -0.21 | ||||
消减值 SBV | 0.19 | 0.78** | -0.78** | -0.20 | -0.94** | 0.31** | ||
0.12 | 0.86** | -0.62** | -0.10 | -0.89** | 0.43** | |||
回复值 CSV | 0.02 | 0.81** | -0.68** | -0.40** | -0.60** | 0.21 | 0.83** | |
0.02 | 0.78** | -0.27* | -0.41** | -0.41** | 0.58** | 0.78** | ||
峰值时间 PeT | 0.07 | 0.46** | -0.17 | 0.45** | -0.72** | 0.69** | 0.64** | 0.34** |
0.02 | 0.38** | -0.27* | 0.41** | -0.41** | 0.58** | 0.78** | 0.92** |
图1 利用Sasanishiki/Habataki BIL群体在2个环境检测到的品质性状QTL的染色体位置 E1表示江苏省南京市; E2表示江苏省金湖县; “+” and “-”分别代表增效等位基因来自于Sasanishiki和Habataki。
Fig. 1. Locations of the identified QTL for rice grain quality using Sasanishiki/Habataki BIL population in two environments. E1, Nanjing, Jiangsu Province, China; E2, Jinhu, Jiangsu Province, China; “+” and “-” following the abbreviation of traits indicate the additive effects of the alleles are contributed by Sasanishiki and Habataki, respectively.
位点 Loci | 染色体 Chr. | 标记区间 Marker interval | 地点 Location | LOD值 LOD value | 贡献率 PVE/% | 加性效应 Effect | 效应来源 Positive allele | ||
---|---|---|---|---|---|---|---|---|---|
qPC1 | 1 | R1485 | - | R886 | E1 | 2.71 | 11.46 | -0.47 | Habataki |
qPC8 | 8 | R2382 | - | S14074 | E1 | 3.26 | 14.44 | 0.45 | Sasanishiki |
E2 | 2.44 | 11.47 | 0.39 | Sasanishiki | |||||
qPC9 | 9 | R1751 | - | G385 | E1 | 2.25 | 10.23 | -0.61 | Habataki |
qPC12.1 | 12 | C1336 | - | R367 | E2 | 3.31 | 13.89 | 0.47 | Sasanishiki |
qPC12.2 | 12 | S1436 | - | R1709 | E2 | 3.35 | 16.16 | -0.44 | Habataki |
qAC4 | 4 | G264 | - | G177 | E1 | 2.91 | 10.69 | -1.16 | Habataki |
E2 | 2.94 | 10.71 | -1.15 | Habataki | |||||
qAC5 | 5 | R3166 | - | R708 | E1 | 2.01 | 7.70 | 1.24 | Sasanishiki |
qAC10 | 10 | R2447 | - | R3285 | E1 | 3.82 | 16.38 | -2.09 | Habataki |
E2 | 4.00 | 16.97 | -2.09 | Habataki | |||||
qPKV2 | 2 | R1843 | - | S2068 | E1 | 2.78 | 22.34 | 26.89 | Sasanishiki |
E2 | 2.99 | 12.67 | 14.48 | Sasanishiki | |||||
qPKV3 | 3 | S1466 | - | R19 | E2 | 2.45 | 9.96 | -11.87 | Habataki |
qPKV4 | 4 | G271 | - | C513 | E2 | 2.20 | 10.13 | 10.51 | Sasanishiki |
qPKV7 | 7 | R2829 | - | R2401 | E1 | 2.10 | 7.72 | 13.69 | Sasanishiki |
E2 | 3.32 | 21.72 | 16.30 | Sasanishiki | |||||
qPKV10 | 10 | R2447 | - | R3285 | E1 | 2.35 | 8.74 | 16.30 | Sasanishiki |
qHPV2 | 2 | C37 | - | C1236 | E2 | 3.35 | 15.32 | 11.53 | Sasanishiki |
qHPV7 | 7 | R2829 | - | R2401 | E1 | 2.87 | 14.66 | 8.65 | Sasanishiki |
E2 | 2.69 | 11.74 | 8.05 | Sasanishiki | |||||
qCPV1 | 1 | C470 | - | R1944 | E1 | 3.10 | 15.38 | 14.15 | Sasanishiki |
E2 | 2.56 | 9.47 | 8.94 | Sasanishiki | |||||
qCPV2 | 2 | G1340 | - | R1843 | E1 | 2.39 | 11.31 | 13.39 | Sasanishiki |
qCPV8 | 8 | R2382 | - | S14074 | E1 | 2.88 | 12.51 | 14.96 | Sasanishiki |
qCPV9 | 9 | C711 | - | G36 | E2 | 2.40 | 15.68 | -9.69 | Habataki |
qCPV10 | 10 | R716 | - | C16 | E1 | 2.38 | 12.82 | 15.08 | Sasanishiki |
qBDV1 | 1 | G393 | - | C813 | E1 | 2.51 | 8.86 | -11.74 | Habataki |
qBDV2 | 2 | R1843 | - | S2068 | E1 | 3.21 | 11.77 | 14.38 | Sasanishiki |
qBDV3 | 3 | C1351 | - | C1468 | E2 | 2.14 | 7.87 | -6.47 | Habataki |
qBDV4 | 4 | G264 | - | G177 | E1 | 2.96 | 10.55 | 12.93 | Sasanishiki |
E2 | 3.02 | 11.45 | 13.04 | Sasanishiki | |||||
qBDV5 | 5 | R3166 | - | R708 | E1 | 2.03 | 7.90 | -11.59 | Habataki |
qBDV7 | 7 | R2829 | - | R2401 | E1 | 2.98 | 17.66 | 8.97 | Sasanishiki |
E2 | 3.24 | 19.36 | 9.43 | Sasanishiki | |||||
qBDV8 | 8 | R2382 | - | S14074 | E1 | 2.82 | 10.45 | -11.47 | Habataki |
qBDV9 | 9 | G36 | - | R1146 | E2 | 5.21 | 22.47 | 12.74 | Sasanishiki |
qSBV2 | 2 | S2068 | - | C499 | E1 | 2.79 | 21.70 | -26.49 | Habataki |
qSBV5 | 5 | R708 | - | R372 | E2 | 2.29 | 9.39 | 11.84 | Sasanishiki |
qSBV7 | 7 | R2829 | - | R2401 | E1 | 3.90 | 14.99 | -19.42 | Habataki |
E2 | 2.84 | 18.41 | -13.72 | Habataki | |||||
qSBV9 | 9 | G36 | - | R1146 | E2 | 2.75 | 29.11 | -18.62 | Habataki |
qSBV10 | 10 | S2083 | - | R2174 | E1 | 2.80 | 10.50 | -17.12 | Habataki |
qSBV12 | 12 | C1336 | - | R367 | E2 | 2.73 | 11.73 | -10.91 | Habataki |
qCSV1 | 1 | R1613 | - | S1778 | E2 | 2.81 | 13.44 | 6.50 | Sasanishiki |
qCSV2 | 2 | S2068 | - | C499 | E1 | 2.13 | 13.66 | -11.18 | Habataki |
qCSV5 | 5 | R708 | - | R372 | E1 | 2.80 | 10.61 | 8.30 | Sasanishiki |
qCSV7 | 7 | R2829 | - | R2401 | E1 | 2.55 | 8.13 | 7.29 | Sasanishiki |
qCSV8 | 8 | R1943 | - | G278 | E1 | 2.15 | 8.13 | 7.29 | Sasanishiki |
qPeT1.1 | 1 | R1613 | - | S1778 | E2 | 2.81 | 13.44 | 6.50 | Sasanishiki |
qPeT1.2 | 1 | G393 | - | C813 | E1 | 2.70 | 12.75 | 0.10 | Sasanishiki |
qPeT8 | 8 | R2382 | - | S14074 | E1 | 3.49 | 20.47 | 0.13 | Sasanishiki |
表 3 BIL群体中检测到的控制稻米品质的QTL
Table 3 QTL affecting rice quality detected in Sasanishiki/Habataki BIL.
位点 Loci | 染色体 Chr. | 标记区间 Marker interval | 地点 Location | LOD值 LOD value | 贡献率 PVE/% | 加性效应 Effect | 效应来源 Positive allele | ||
---|---|---|---|---|---|---|---|---|---|
qPC1 | 1 | R1485 | - | R886 | E1 | 2.71 | 11.46 | -0.47 | Habataki |
qPC8 | 8 | R2382 | - | S14074 | E1 | 3.26 | 14.44 | 0.45 | Sasanishiki |
E2 | 2.44 | 11.47 | 0.39 | Sasanishiki | |||||
qPC9 | 9 | R1751 | - | G385 | E1 | 2.25 | 10.23 | -0.61 | Habataki |
qPC12.1 | 12 | C1336 | - | R367 | E2 | 3.31 | 13.89 | 0.47 | Sasanishiki |
qPC12.2 | 12 | S1436 | - | R1709 | E2 | 3.35 | 16.16 | -0.44 | Habataki |
qAC4 | 4 | G264 | - | G177 | E1 | 2.91 | 10.69 | -1.16 | Habataki |
E2 | 2.94 | 10.71 | -1.15 | Habataki | |||||
qAC5 | 5 | R3166 | - | R708 | E1 | 2.01 | 7.70 | 1.24 | Sasanishiki |
qAC10 | 10 | R2447 | - | R3285 | E1 | 3.82 | 16.38 | -2.09 | Habataki |
E2 | 4.00 | 16.97 | -2.09 | Habataki | |||||
qPKV2 | 2 | R1843 | - | S2068 | E1 | 2.78 | 22.34 | 26.89 | Sasanishiki |
E2 | 2.99 | 12.67 | 14.48 | Sasanishiki | |||||
qPKV3 | 3 | S1466 | - | R19 | E2 | 2.45 | 9.96 | -11.87 | Habataki |
qPKV4 | 4 | G271 | - | C513 | E2 | 2.20 | 10.13 | 10.51 | Sasanishiki |
qPKV7 | 7 | R2829 | - | R2401 | E1 | 2.10 | 7.72 | 13.69 | Sasanishiki |
E2 | 3.32 | 21.72 | 16.30 | Sasanishiki | |||||
qPKV10 | 10 | R2447 | - | R3285 | E1 | 2.35 | 8.74 | 16.30 | Sasanishiki |
qHPV2 | 2 | C37 | - | C1236 | E2 | 3.35 | 15.32 | 11.53 | Sasanishiki |
qHPV7 | 7 | R2829 | - | R2401 | E1 | 2.87 | 14.66 | 8.65 | Sasanishiki |
E2 | 2.69 | 11.74 | 8.05 | Sasanishiki | |||||
qCPV1 | 1 | C470 | - | R1944 | E1 | 3.10 | 15.38 | 14.15 | Sasanishiki |
E2 | 2.56 | 9.47 | 8.94 | Sasanishiki | |||||
qCPV2 | 2 | G1340 | - | R1843 | E1 | 2.39 | 11.31 | 13.39 | Sasanishiki |
qCPV8 | 8 | R2382 | - | S14074 | E1 | 2.88 | 12.51 | 14.96 | Sasanishiki |
qCPV9 | 9 | C711 | - | G36 | E2 | 2.40 | 15.68 | -9.69 | Habataki |
qCPV10 | 10 | R716 | - | C16 | E1 | 2.38 | 12.82 | 15.08 | Sasanishiki |
qBDV1 | 1 | G393 | - | C813 | E1 | 2.51 | 8.86 | -11.74 | Habataki |
qBDV2 | 2 | R1843 | - | S2068 | E1 | 3.21 | 11.77 | 14.38 | Sasanishiki |
qBDV3 | 3 | C1351 | - | C1468 | E2 | 2.14 | 7.87 | -6.47 | Habataki |
qBDV4 | 4 | G264 | - | G177 | E1 | 2.96 | 10.55 | 12.93 | Sasanishiki |
E2 | 3.02 | 11.45 | 13.04 | Sasanishiki | |||||
qBDV5 | 5 | R3166 | - | R708 | E1 | 2.03 | 7.90 | -11.59 | Habataki |
qBDV7 | 7 | R2829 | - | R2401 | E1 | 2.98 | 17.66 | 8.97 | Sasanishiki |
E2 | 3.24 | 19.36 | 9.43 | Sasanishiki | |||||
qBDV8 | 8 | R2382 | - | S14074 | E1 | 2.82 | 10.45 | -11.47 | Habataki |
qBDV9 | 9 | G36 | - | R1146 | E2 | 5.21 | 22.47 | 12.74 | Sasanishiki |
qSBV2 | 2 | S2068 | - | C499 | E1 | 2.79 | 21.70 | -26.49 | Habataki |
qSBV5 | 5 | R708 | - | R372 | E2 | 2.29 | 9.39 | 11.84 | Sasanishiki |
qSBV7 | 7 | R2829 | - | R2401 | E1 | 3.90 | 14.99 | -19.42 | Habataki |
E2 | 2.84 | 18.41 | -13.72 | Habataki | |||||
qSBV9 | 9 | G36 | - | R1146 | E2 | 2.75 | 29.11 | -18.62 | Habataki |
qSBV10 | 10 | S2083 | - | R2174 | E1 | 2.80 | 10.50 | -17.12 | Habataki |
qSBV12 | 12 | C1336 | - | R367 | E2 | 2.73 | 11.73 | -10.91 | Habataki |
qCSV1 | 1 | R1613 | - | S1778 | E2 | 2.81 | 13.44 | 6.50 | Sasanishiki |
qCSV2 | 2 | S2068 | - | C499 | E1 | 2.13 | 13.66 | -11.18 | Habataki |
qCSV5 | 5 | R708 | - | R372 | E1 | 2.80 | 10.61 | 8.30 | Sasanishiki |
qCSV7 | 7 | R2829 | - | R2401 | E1 | 2.55 | 8.13 | 7.29 | Sasanishiki |
qCSV8 | 8 | R1943 | - | G278 | E1 | 2.15 | 8.13 | 7.29 | Sasanishiki |
qPeT1.1 | 1 | R1613 | - | S1778 | E2 | 2.81 | 13.44 | 6.50 | Sasanishiki |
qPeT1.2 | 1 | G393 | - | C813 | E1 | 2.70 | 12.75 | 0.10 | Sasanishiki |
qPeT8 | 8 | R2382 | - | S14074 | E1 | 3.49 | 20.47 | 0.13 | Sasanishiki |
QTL位点 Target QTL | 目标置换系 CSSLs | 南京市 Nanjing, Jiangsu Province | 金湖县 Jinhu, Jiangsu Province | |||
---|---|---|---|---|---|---|
平均值 Mean | P值 P-value | 平均值 Mean | P值 P-value | |||
qPC8 | Sasanishiki | 7.15 | 7.36 | |||
SL428 | 6.58 | 0.003** | 6.62 | 0.001** | ||
qAC4 | Sasanishiki | 14.18 | 14.32 | |||
SL414 | 13.15 | 0.004** | 13.06 | 0.002** | ||
qAC10 | Sasanishiki | 14.18 | 14.32 | |||
SL431 | 11.43 | 0.002** | 11.56 | 0.005** | ||
SL432 | 14.42 | 0.030* | 15.38 | 0.010** | ||
qPKV2 | Sasanishiki | 362.75 | 301.83 | |||
SL406 | 208.70 | 0.004** | 155.08 | 0.005** | ||
SL407 | 197.40 | 0.008** | 153.83 | 0.002** | ||
qPKV7 | Sasanishiki | 362.75 | 301.83 | |||
SL422 | 393.50 | 0.005** | 345.60 | 0.004** | ||
qHPV7 | Sasanishiki | 229.75 | 134.58 | 0.003** | ||
SL422 | 322.50 | 0.004** | 210.50 | 0.008** | ||
qCPV1 | Sasanishiki | 326.67 | 235.75 | |||
SL401 | 367.50 | 0.020* | 257.80 | 0.030* | ||
qBDV4 | Sasanishiki | 133.00 | 167.25 | |||
SL414 | 178.50 | 0.007** | 200.60 | 0.006** | ||
qBDV7 | Sasanishiki | 133.00 | 167.25 | |||
SL422 | 71.00 | 0.002** | 135.10 | 0.005** | ||
qSBV7 | Sasanishiki | -36.08 | -66.08 | |||
SL422 | -16.50 | 0.009** | -47.60 | 0.006** |
表4 稻米品质性状QTL对应置换系与背景亲本Sasanishiki在2个环境中的表型差异
Table 4 Phenotypic differences between genetic background parent Sasanishiki and target CSSLs carrying rice quality traits QTLs across two environments.
QTL位点 Target QTL | 目标置换系 CSSLs | 南京市 Nanjing, Jiangsu Province | 金湖县 Jinhu, Jiangsu Province | |||
---|---|---|---|---|---|---|
平均值 Mean | P值 P-value | 平均值 Mean | P值 P-value | |||
qPC8 | Sasanishiki | 7.15 | 7.36 | |||
SL428 | 6.58 | 0.003** | 6.62 | 0.001** | ||
qAC4 | Sasanishiki | 14.18 | 14.32 | |||
SL414 | 13.15 | 0.004** | 13.06 | 0.002** | ||
qAC10 | Sasanishiki | 14.18 | 14.32 | |||
SL431 | 11.43 | 0.002** | 11.56 | 0.005** | ||
SL432 | 14.42 | 0.030* | 15.38 | 0.010** | ||
qPKV2 | Sasanishiki | 362.75 | 301.83 | |||
SL406 | 208.70 | 0.004** | 155.08 | 0.005** | ||
SL407 | 197.40 | 0.008** | 153.83 | 0.002** | ||
qPKV7 | Sasanishiki | 362.75 | 301.83 | |||
SL422 | 393.50 | 0.005** | 345.60 | 0.004** | ||
qHPV7 | Sasanishiki | 229.75 | 134.58 | 0.003** | ||
SL422 | 322.50 | 0.004** | 210.50 | 0.008** | ||
qCPV1 | Sasanishiki | 326.67 | 235.75 | |||
SL401 | 367.50 | 0.020* | 257.80 | 0.030* | ||
qBDV4 | Sasanishiki | 133.00 | 167.25 | |||
SL414 | 178.50 | 0.007** | 200.60 | 0.006** | ||
qBDV7 | Sasanishiki | 133.00 | 167.25 | |||
SL422 | 71.00 | 0.002** | 135.10 | 0.005** | ||
qSBV7 | Sasanishiki | -36.08 | -66.08 | |||
SL422 | -16.50 | 0.009** | -47.60 | 0.006** |
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